🔍
Milk based drinks

What is acidophilus milk and how is it made?

Quick answer

Acidophilus milk is milk containing Lactobacillus acidophilus cultures. Commercial sweet acidophilus milk is sold as regular drinking milk with added cultures, while the cultured home version is made by heating milk, cooling it to about 104 to 113 degrees F and incubating it with a starter for 12 to 24 hours until it thickens and turns tangy.

Free download

Milk and Dairy Storage Chart (PDF): How long milk, plant milks, cream, yogurt and kefir last, opened and unopened. 23 rows, each with its source.

We use your email only for this download and, if you tick the box, to tell you about the pack. No spam. We do not sell it. Delete it any time: contact@drinkstype.com. Privacy policy

Thank you

Your chart is ready

Download the PDF

We use your email only for this download.
In this article
  1. What is acidophilus milk?
  2. How is cultured acidophilus milk made at home?
  3. How is it different from yogurt and kefir?
  4. Is it a health food?

What is acidophilus milk?

It is milk with Lactobacillus acidophilus, a lactic acid bacterium, added. The name covers two kinds of products. Sweet acidophilus milk is sold in cartons like ordinary milk; for example, Purity Dairies markets a fat-free milk with added acidophilus and bifidum cultures. The cultured version is fermented, thicker and tangier, and can be used like a yogurt alternative.

How is cultured acidophilus milk made at home?

Utah State University Extension gives this method for one liter of whole milk:

  1. Heat the milk to 185 degrees F and hold for 10 to 20 minutes, stirring.
  2. Cool to 104 to 113 degrees F.
  3. Stir in one packet of acidophilus starter culture.
  4. Pour into clean jars and hold at 104 to 113 degrees F for 12 to 24 hours, until the pH falls below 4.6.
  5. Refrigerate, then use within one week. Save about 1 cup each week to start the next batch.

USU’s directions for later batches use 1 cup of the saved culture per gallon of milk and 5 to 8 hours of incubation.

How is it different from yogurt and kefir?

Product Typical culture
Acidophilus milk Lactobacillus acidophilus
Yogurt Usually Streptococcus thermophilus and Lactobacillus bulgaricus
Kefir Kefir grains or culture, a mix of bacteria and yeasts

Is it a health food?

Probiotic effects are strain-specific, and official bodies say the best strains and doses are not known for many uses. Treat it as a milk or cultured dairy product, not a treatment. People with weakened immunity should ask a health care provider first.

People also ask

Is acidophilus milk the same as yogurt?

No. Yogurt usually uses different cultures, and sweet acidophilus milk is sold as a drinking milk.

Does acidophilus milk taste sour?

The cultured home version is tangy. Sweet acidophilus milk is sold like ordinary milk, so check the label.

How long does homemade acidophilus milk keep?

USU advises using the re-cultured milk within one week in the refrigerator.

General information only, not medical advice. If you have a health condition or are pregnant, ask your doctor before changing what you drink.

Sources

  1. Utah State University Extension: Acidophilus milk, read 6 Nov 2026
  2. Dairy Foods: Purity rolls out a fat-free acidophilus milk, read 6 Nov 2026
  3. NCCIH: Probiotics, usefulness and safety, read 6 Nov 2026
Written by Drinks Type Editorial Team · Published Dec 3, 2025 · Updated Nov 6, 2026
About · How we verify this · Editorial policy · Corrections

What would help you most next?

One tap. Anonymous. It helps us decide what to build.

More in Milk based drinks

All Milk based drinks ›
Milk based drinks

Is sour cream gluten-free?

Is sour cream naturally gluten-free? Gluten comes from wheat, barley and rye. The NHS coeliac disease page lists “most dairy products, such as cheese,…

By Drinks Type Editorial Team · Updated Nov 2026